This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
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What are the best things to buy during Amazon's Big Spring sale?An Amazon sale means some of the best savings will be on Amazon devices like Echos, Fire TVs, and Kindles, but the reach of its spring sale goes far beyond that. With the focus on the spring season, expect to see lots of savings on outdoor gear like furniture, grills, tents, and power stations. Plus, there will be plenty of classic big sale savings on TVs, laptops, headphones, and tablets.
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